產(chǎn)品詳細(xì)介紹
E.Z.N.A.® Plasmid Mini Kit I |
從1-5ml過(guò)夜培養(yǎng)的菌液中純化高達(dá)35µg質(zhì)粒DNA |
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該試劑盒采用經(jīng)典的堿裂解法和硅膠柱純化方式,適合于從1.5-5.0ml培養(yǎng)過(guò)夜的大腸桿菌(DH5α, JM109)等菌液中抽提5-35µg高純度的質(zhì)粒DNA。操作者可在30分鐘內(nèi)完成多個(gè)樣品的質(zhì)粒提取工作。純化的質(zhì)粒可以直接用于自動(dòng)測(cè)序、限制性內(nèi)切酶酶切、PCR、標(biāo)記等實(shí)驗(yàn)。從1.5-5.0ml培養(yǎng)過(guò)夜的菌液中收集細(xì)菌后,采用經(jīng)典的堿裂解法裂解細(xì)菌,離心去除染色體DNA和蛋白質(zhì),上清液轉(zhuǎn)移至硅膠柱,離心吸附質(zhì)粒DNA,經(jīng)兩種溶液洗滌去除殘余蛋白質(zhì)和各種雜質(zhì),最后用Elution Buffer洗脫出質(zhì)粒DNA。 |
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產(chǎn)品特性與優(yōu)點(diǎn) |
- 安全-無(wú)酚氯仿等危險(xiǎn)的有機(jī)物抽提
- 簡(jiǎn)單-硅膠柱過(guò)濾操作
- 快速-在30分鐘內(nèi)完成多個(gè)樣品的抽提工作
- 超值-性價(jià)比最高
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產(chǎn)品鏈接 |
英文說(shuō)明書(shū)
中文說(shuō)明書(shū)
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定購(gòu)信息 |
編碼 |
名稱 |
次數(shù) |
詳情 |
單價(jià) |
D6942-00 |
Plasmid Mini Kit I, Q-Spin Column |
5 |
Solution I, Solution II, Solution III, Buffer HB, DNA Wash Buffer, Elution Buffer, RNase A, HiBind DNA Column I (5), 2 ml Collection Tubes(5) |
50.00 |
D6942-01 |
Plasmid Mini Kit I, Q-Spin Column |
50 |
Solution I, Solution II, Solution III, Buffer HB, DNA Wash Buffer, Elution Buffer, RNase A, HiBind DNA Column I (50), 2 ml Collection Tubes(50) |
199.00 |
D6942-02 |
Plasmid Mini Kit I, Q-Spin Column |
200 |
Solution I, Solution II, Solution III, Buffer HB, DNA Wash Buffer, Elution Buffer, RNase A, HiBind DNA Column I (200), 2 ml Collection Tubes(200) |
680.00 |
D6943-00 |
Plasmid Mini Kit I, V-Spin Column |
5 |
Solution I, Solution II, Solution III, Buffer HB, DNA Wash Buffer, Elution Buffer, RNase A, HiBind DNA Column I (5), 2 ml Collection Tubes(5) |
50.00 |
D6943-01 |
Plasmid Mini Kit I, V-Spin Column |
50 |
Solution I, Solution II, Solution III, Buffer HB, DNA Wash Buffer, Elution Buffer, RNase A, HiBind DNA Column I (50), 2 ml Collection Tubes(50) |
199.00 |
D6943-02 |
Plasmid Mini Kit I, V-Spin Column |
200 |
Solution I, Solution II, Solution III, Buffer HB, DNA Wash Buffer, Elution Buffer, RNase A, HiBind DNA Column I (200), 2 ml Collection Tubes(200) |
680.00 |
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產(chǎn)品編號(hào) |
產(chǎn)品名稱 |
產(chǎn)品包裝 |
產(chǎn)品價(jià)格 |
D6942-01 |
質(zhì)粒小量提取試劑盒 I 型 |
100T |
¥299 |
D6945-01 |
質(zhì)粒小量提取試劑盒 II型 |
50T |
¥495.00 |
D6904-01 |
質(zhì)粒中量提取試劑盒 |
10T |
¥500.00 |
D6922-02 |
質(zhì)粒大量提取試劑盒 |
20T |
¥1350.00 |
D6905-03 |
質(zhì)粒快速中量提取試劑盒 |
25T |
¥1350.00 |
D6924-01 |
質(zhì)?焖俅罅刻崛≡噭┖ |
5T |
¥405.00 |
D6929-00 |
快速過(guò)濾超大量質(zhì)粒提取試劑盒 |
2T |
¥580.00 |
D1097-01 |
96孔板快速過(guò)濾質(zhì)粒提取試劑盒 |
4 x 96 |
¥2000.00 |
D6920-00 |
宏量質(zhì)粒提取試劑盒 |
2T |
¥845.00 |
D1095-00 |
96孔板簡(jiǎn)單經(jīng)濟(jì)型質(zhì)粒提取試劑盒 |
1 x 96 |
¥400.00 |
D3376-01 |
酵母質(zhì)粒提取試劑盒 |
50T |
¥495.00 |
D6947-01 |
超快速質(zhì)粒小量提取試劑盒 |
50T |
¥435.00 |
D1047-00 |
超快速質(zhì)粒小量提取試劑 |
1 x 96 |
¥1060.00 |
D1532-00 |
96孔板S質(zhì)粒提取試劑盒 |
1 x 96 |
¥630.00 |
D6948-01 |
無(wú)內(nèi)毒素小量質(zhì)粒提取試劑盒 I |
50T |
¥540.00 |
D6950-01 |
無(wú)內(nèi)毒素小量質(zhì)粒提取試劑盒 II |
50T |
¥675.00 |
D6915-01 |
無(wú)內(nèi)毒素中/大量提取試劑盒 |
10T |
¥630.00 |
D6926-01 |
無(wú)內(nèi)毒素中/大量提取試劑盒 |
6T |
¥594.00 |
D6234-00 |
無(wú)內(nèi)毒素超大量質(zhì)粒提取試劑盒 |
2T |
¥1304.00 |
D7043-00 |
質(zhì)粒高純(高性能)小量提取試劑盒 |
50T |
¥484.00 |
D7045-01 |
質(zhì)粒高純(高性能)小量提取試劑盒 |
50T |
¥585.00 |
D7004-01 |
質(zhì)粒高純高性能中/大量提取試劑盒 |
10T |
¥585.00 |
D7022-01 |
質(zhì)粒高純高性能中/大量提取試劑盒 |
5T |
¥675.00 |
D2156-01 |
BAC/PAC大型質(zhì)粒提取試劑盒 |
50T |
¥484.00 |
D1055-01 |
BAC/PAC大型質(zhì)粒提取試劑盒 |
4 x 96 |
¥2500.00 |
D2154-00 |
BAC/PAC 大型質(zhì)粒小提/大提試劑盒 |
2T |
¥305.00 |
D6908-00 |
m-13單鏈DNA小提/大提/96孔板提取試劑盒 |
2T |
¥326.00 |
D1900-01 |
M-13單鏈DNA純化試劑盒 |
4 x 96 |
¥3150 |
M1257-01 |
磁珠質(zhì)粒大量提取試劑盒 |
5T |
¥621.00 |
M1259-01 |
磁珠質(zhì)粒超大量提取試劑盒 |
5T |
¥1100.00 |
M1256-01 |
96孔磁珠質(zhì)粒提取試劑盒 |
4 x 96 |
¥2484.00 |
M1258-01 |
磁珠無(wú)內(nèi)毒素小量提取試劑盒 |
4x96 |
¥3850.00 |
M1252-00 |
磁珠無(wú)內(nèi)毒素大量提取試劑盒 |
2T |
¥360.00 |
M1253-00 |
磁珠無(wú)內(nèi)毒素提取試劑盒 |
2T |
¥1987.00 |
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